Reconstitution Guide
What you need
- The vial of lyophilized powder.
- Bacteriostatic water as solvent (sterile water with 0.9% benzyl alcohol).
- A sterile graduated syringe or pipette to measure and transfer the solvent.
- Alcohol wipes (70% isopropyl alcohol) to clean the rubber stoppers.
- Gloves and a clean workspace.
Individual lab supplies can be found in Lab accessories.
The procedure in five steps
01 — Preparation
Prepare everything before you start and allow the vial to reach room temperature.
Lab tip: cold glass causes condensation inside the vial; twenty minutes on the workbench is usually sufficient.
02 — Cleaning stoppers
Clean the rubber stopper of the peptide vial and the solvent vial with an alcohol wipe and allow them to dry.
Lab tip: do not touch the cleaned stopper afterward.
03 — Adding solvent
Draw the calculated volume of bacteriostatic water from the vial and let it flow slowly along the glass wall into the vial, not directly onto the powder.
Lab tip: the calculator calculates how much solvent you need for the desired concentration.
04 — Dissolving
Swirl gently until the powder has completely dissolved. Do not shake. The solution should be clear.
Lab tip: if the solution remains cloudy or you observe flocculation, note down the lot number and contact us.
05 — Labeling and storage
Write the date and the concentration on the label. Store the solution at 2–8 °C, protected from light, and use it within the timeframe specified for the product. Store unopened, lyophilized vials at −20 °C.
Lab tip: in three weeks, you won't remember the concentration from memory.
Common mistakes
- Forcefully ejecting the solvent onto the powder.
- Shaking instead of swirling.
- Using tap water or water without preservative for a solution intended for longer storage.
- Returning a reconstituted vial to the freezer.